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empty vectors pgadt7  (TaKaRa)


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    TaKaRa empty vectors pgadt7
    Empty Vectors Pgadt7, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 3955 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/empty vectors pgadt7/product/TaKaRa
    Average 99 stars, based on 3955 article reviews
    empty vectors pgadt7 - by Bioz Stars, 2026-02
    99/100 stars

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    Analysis of the interaction between LrWRKY3 and LrDef1 promoter. a EMSA revealed that the LrWRKY3 specifically bound to W box. 1, reaction solution containing only free probes; 2, LrWRKY3 can interact with biotin labelled probes containing the W box sequence; 3, LrWRKY3 can both interact with biotin labelled probes containing the W box sequence and unlabeled probes containing the W box sequence; 4, LrWRKY3 can’t interact with mutant probes; Complex: the combination of LrWRKY3 recombinant protein and probes; Free probe: the unbound probes. b Analysis of the transactivation effect of LrWRKY3 on LrDef1 promoter. LrWRKY3 + pLrDef1 : transcriptional activity analysis of <t>pGADT7-</t> LrWRKY3 interaction with pLrDef1 ; Positive control: pGADT7- p53 can interact with pAbAi- p53 as a positive control; Negative control: pAbAi empty vector was used a negative control. c GUS activity was higher in transgenic tobacco lines co-expressing of LrWRKY3 and LrDef1 promoter than in the transgenic tobacco lines expressing of LrDef1 promoter. The results were shown as average values calculated from three replicates and calculated by the 2 -ΔΔCt method and analyzed by the Student’s t test. pBI121: pBI121- GUS transgenic tobacco; PD-2/3/7/10: pLrDef1 transgenic tobacco lines. Co-1/7/9/12: LrWRKY3 / pLrDef1 transgenic tobacco lines
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    Analysis of the interaction between LrWRKY3 and LrDef1 promoter. a EMSA revealed that the LrWRKY3 specifically bound to W box. 1, reaction solution containing only free probes; 2, LrWRKY3 can interact with biotin labelled probes containing the W box sequence; 3, LrWRKY3 can both interact with biotin labelled probes containing the W box sequence and unlabeled probes containing the W box sequence; 4, LrWRKY3 can’t interact with mutant probes; Complex: the combination of LrWRKY3 recombinant protein and probes; Free probe: the unbound probes. b Analysis of the transactivation effect of LrWRKY3 on LrDef1 promoter. LrWRKY3 + pLrDef1 : transcriptional activity analysis of <t>pGADT7-</t> LrWRKY3 interaction with pLrDef1 ; Positive control: pGADT7- p53 can interact with pAbAi- p53 as a positive control; Negative control: pAbAi empty vector was used a negative control. c GUS activity was higher in transgenic tobacco lines co-expressing of LrWRKY3 and LrDef1 promoter than in the transgenic tobacco lines expressing of LrDef1 promoter. The results were shown as average values calculated from three replicates and calculated by the 2 -ΔΔCt method and analyzed by the Student’s t test. pBI121: pBI121- GUS transgenic tobacco; PD-2/3/7/10: pLrDef1 transgenic tobacco lines. Co-1/7/9/12: LrWRKY3 / pLrDef1 transgenic tobacco lines
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    Analysis of the interaction between LrWRKY3 and LrDef1 promoter. a EMSA revealed that the LrWRKY3 specifically bound to W box. 1, reaction solution containing only free probes; 2, LrWRKY3 can interact with biotin labelled probes containing the W box sequence; 3, LrWRKY3 can both interact with biotin labelled probes containing the W box sequence and unlabeled probes containing the W box sequence; 4, LrWRKY3 can’t interact with mutant probes; Complex: the combination of LrWRKY3 recombinant protein and probes; Free probe: the unbound probes. b Analysis of the transactivation effect of LrWRKY3 on LrDef1 promoter. LrWRKY3 + pLrDef1 : transcriptional activity analysis of pGADT7- LrWRKY3 interaction with pLrDef1 ; Positive control: pGADT7- p53 can interact with pAbAi- p53 as a positive control; Negative control: pAbAi empty vector was used a negative control. c GUS activity was higher in transgenic tobacco lines co-expressing of LrWRKY3 and LrDef1 promoter than in the transgenic tobacco lines expressing of LrDef1 promoter. The results were shown as average values calculated from three replicates and calculated by the 2 -ΔΔCt method and analyzed by the Student’s t test. pBI121: pBI121- GUS transgenic tobacco; PD-2/3/7/10: pLrDef1 transgenic tobacco lines. Co-1/7/9/12: LrWRKY3 / pLrDef1 transgenic tobacco lines

    Journal: BMC Plant Biology

    Article Title: Lilium regale Wilson WRKY3 modulates an antimicrobial peptide gene, LrDef1 , during response to Fusarium oxysporum

    doi: 10.1186/s12870-022-03649-y

    Figure Lengend Snippet: Analysis of the interaction between LrWRKY3 and LrDef1 promoter. a EMSA revealed that the LrWRKY3 specifically bound to W box. 1, reaction solution containing only free probes; 2, LrWRKY3 can interact with biotin labelled probes containing the W box sequence; 3, LrWRKY3 can both interact with biotin labelled probes containing the W box sequence and unlabeled probes containing the W box sequence; 4, LrWRKY3 can’t interact with mutant probes; Complex: the combination of LrWRKY3 recombinant protein and probes; Free probe: the unbound probes. b Analysis of the transactivation effect of LrWRKY3 on LrDef1 promoter. LrWRKY3 + pLrDef1 : transcriptional activity analysis of pGADT7- LrWRKY3 interaction with pLrDef1 ; Positive control: pGADT7- p53 can interact with pAbAi- p53 as a positive control; Negative control: pAbAi empty vector was used a negative control. c GUS activity was higher in transgenic tobacco lines co-expressing of LrWRKY3 and LrDef1 promoter than in the transgenic tobacco lines expressing of LrDef1 promoter. The results were shown as average values calculated from three replicates and calculated by the 2 -ΔΔCt method and analyzed by the Student’s t test. pBI121: pBI121- GUS transgenic tobacco; PD-2/3/7/10: pLrDef1 transgenic tobacco lines. Co-1/7/9/12: LrWRKY3 / pLrDef1 transgenic tobacco lines

    Article Snippet: Moreover, the yeast prey empty vector pGADT7 AD was transferred into bait strain pAbAi- pLrDef1- Y1HGold competent cells as a negative control, and the pGADT7 AD- P53 vector (kit provided, Takara) was transferred into bait vector pAbAi- p53 -Y1HGOLD competent cells as a positive control.

    Techniques: Sequencing, Mutagenesis, Recombinant, Activity Assay, Positive Control, Negative Control, Plasmid Preparation, Transgenic Assay, Expressing